Isolation and screening of amylases, pectinases and cellulases producing fungi from organic wastes


  • Asmina Noureen Lahore Garrison University
  • Shomaila Sikandar Lahore Garrison University
  • Imran Afzal Lahore Garrison University
  • Sadaf Sarfraz Lahore Garrison University


Hydrolytic enzyme, Plate assay method, Indigenous fungi


Filamentous fungi are ubiquitous in nature and produced wide range of industrially important extracellular enzymes. The main focus of the present study is the isolation of indigenous fungi capable of producing extracellular enzymes. In present study, total thirteen fungal isolates were isolated from different samples including; soil samples, organic waste, vegetables, dry fruits and tamarind bark. The fungal isolates were further screened for their amylolytic, cellulytic and pectinolytic activities on their selective media by using plate assay method. Among thirteen fungal isolates, four isolates gave maximum pectinolytic and cellulolytic activities, while only three fungal isolates gave highest amylolytic activity in qualitative analysis. Furthermore, potential fungal isolates were identified on the basis of colony morphology and microscopy. The identified fungal isolates were Cladosporium sp., Fusarium sp., Curvulari sp., and Trichoderma sp. The present study found huge applications of hydrolytic enzymes produced from indigenous fungi in different industries like food beverages, textile, paper and pulp.