Antioxidant Assessment of Mentha spicata Leaf Extract And GC-MS Profiling of Metabolite

Abstract

Mentha spicata is a member of Lamiaceae family and containing diverse array of secondary metabolites, having significant role in medicinal contexts. The importance of the secondary metabolites of the M. spicata is widely acknowledged; however, some metabolites need further assessment to determine their potential applications in medicine. In the present study, the metabolites are extracted from the M. spicata using ethanol and are analyzed through Gas Chromatography-Mass Spectrometry (GC-MS). The list of metabolites such as Undecane, Hexadecanoic acid methyl ester, 12,15-Octadecadienoic acid methyl ester, 6-Octadecenoic acid methyl ester (Z), 6-Octadecenoic acid methyl ester, Cyclohexane, methyl ester, Phenol, 4,4'-(1-methylethylidene) bis, Sulfurous acid cyclohexylmethyl dodecyl ester, Octasiloxane -hexadecamethyl are identified in the extract. The antioxidant efficacy of the extract is evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays. Results show that at a concentration of 1 mg/mL, scavenging activity of the extract is 20 ± 2%, which is enhanced in a dose dependent manner by increasing the extract concentration. Concentrations 2 mg/mL and 6 mg/mL result the activities up to 22.4 ± 2% to 28.6 ± 2.5. GC-MS analysis facilitates identification of metabolites, improving the knowledge about their nature and antioxidant properties and DPPH assay highlights to scavenge free radicals. Both GC-MS and DPPH assay highlight the role of the M. spicata in reducing oxidative stress and its potential applications in pharmaceuticals.