Metabolite Profiling by GC-MS and Antioxidant Properties of Punica granatum

Abstract

Punica granatum has a great medicinal use due to a wide range of secondary metabolites which have very important roles in cell signaling immunity, and reproduction. In the present study, metabolites from P. granatum are extracted using 75% ethanol and dehydrated using a rotatory. The metabolites are identified through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The resultant metabolites in the ethanolic extract are including Undecane, 3,7-dimethyl- Hexadecanoic acid methyl ester, 11,14-Octadecadienoic acid methyl ester, 6-Octadecenoic acid methyl ester, 9-Octadecenoic acid methyl ester, Methyl stearate, Phenol 4,4'-(1-methylethylidene), Naphthalene-1-sulfonic acid 4-methoxy-,(2-adamantan-1-ylethyl)amide, Silane, [(10-iododecyl)oxy]trimethyl, and Cyclodecasiloxane, eicosamethyl. The metabolites are assessed based on peak area, retention time and height of the peak and are compared standard reference compounds from NIST libraries. Moreover, antioxidant properties of the extract are evaluated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays. The DPPH results revealed a dose-dependent increase in activity from 18 ± 2% to 25% as concentrations are increased from at 100 μg/ml to 700 μg/ml. these results suggested the potential health benefits and antioxidant properties of P. granatum metabolites. However, further studies are recommended to explore the in-depth medicinal potential of the P. granatum.